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THRA Monoclonal antibody

THRA Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A11C8

Cat No : 66703-1-Ig

Print datasheet

Synonyms

AR7, c ERBA 1, c erbA alpha, EAR 7, EAR7, ERB T 1, ERBA, ERBA1, NR1A1, THRA, THRA1, THRA2, Thyroid hormone receptor alpha, V erbA related protein 7

验证数据展示

  • WB analysis of A431 using 66703-1-Ig

    WB analysis of A431 using 66703-1-Ig

    WB result of THRA antibody (66703-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-THRA transfected A431 cells.

  • IHC staining of mouse brain using 66703-1-Ig

    IHC staining of mouse brain using 66703-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with THRA antibody included
  • IHC staining of mouse brain using 66703-1-Ig

    IHC staining of mouse brain using 66703-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with THRA antibody included
  • IHC staining of human thyroid cancer using 66703-1-Ig

    IHC staining of human thyroid cancer using 66703-1-Ig

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with THRA antibody included
  • IHC staining of mouse heart using 66703-1-Ig

    IHC staining of mouse heart using 66703-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with THRA antibody included
  • IHC staining of rat colon using 66703-1-Ig

    IHC staining of rat colon using 66703-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with THRA antibody included
  • IHC staining of rat heart using 66703-1-Ig

    IHC staining of rat heart using 66703-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66703-1-Ig (THRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with THRA antibody included

经过测试的应用

Positive WB detected inA431 cells
Positive IHC detected inmouse brain tissue, human thyroid cancer tissue, mouse heart tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy THRA Ready-To-Use IHC Kit. THRA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66703-1-Ig targets THRA in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen THRA fusion protein Ag27189 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian)
Synonyms AR7, c ERBA 1, c erbA alpha, EAR 7, EAR7, ERB T 1, ERBA, ERBA1, NR1A1, THRA, THRA1, THRA2, Thyroid hormone receptor alpha, V erbA related protein 7
Calculated Molecular Weight 54 aa, 7 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC002728
Gene Symbol THRA
Gene ID (NCBI) 7067
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10827
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for THRA antibody 66703-1-IgDownload protocol
IHC protocol for THRA antibody 66703-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols