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验证数据展示

  • WB analysis using 11738-1-AP

    WB analysis using 11738-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11738-1-AP (THOP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11738-1-AP

    IHC staining of human colon cancer using 11738-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11738-1-AP (THOP1 antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis using 11738-1-AP

    WB analysis using 11738-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11738-1-AP (THOP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of human placenta using 11738-1-AP

    IP experiment of human placenta using 11738-1-AP

    IP result of anti-THOP1 (IP:11738-1-AP, 4ug; Detection:11738-1-AP 1:500) with human placenta tissue lysate 1520ug.

THOP1 Polyclonal antibody

THOP1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11738-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

Endopeptidase 24.15, EP24.15, MEPD_HUMAN, MP78, Thimet oligopeptidase, thimet oligopeptidase 1, THOP1, TOP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, K-562 cells, human placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11738-1-AP targets THOP1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 THOP1 fusion protein Ag2268 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thimet oligopeptidase 1
别名 Endopeptidase 24.15, EP24.15, MEPD_HUMAN, MP78, Thimet oligopeptidase, thimet oligopeptidase 1, THOP1, TOP
计算分子量 689 aa, 79 kDa
观测分子量 79 kDa
GenBank蛋白编号BC000135
基因名称 THOP1
Gene ID (NCBI) 7064
RRIDAB_2303269
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52888
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Thimet oligopeptidase(THOP1) is a metallopeptidase implicated in the metabolism of many sleep-related peptides, including angiotensin I, gonadotropin releasing hormone (GnRH), neurotensin, and opioid peptides

实验方案

Product Specific Protocols
WB protocol for THOP1 antibody 11738-1-APDownload protocol
IHC protocol for THOP1 antibody 11738-1-APDownload protocol
IP protocol for THOP1 antibody 11738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols