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验证数据展示

  • WB analysis of HeLa using 55178-1-AP

    WB analysis of HeLa using 55178-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 55178-1-AP

    IP experiment of HeLa using 55178-1-AP

    IP result of anti-THOC2 (IP:55178-1-AP, 4ug; Detection:55178-1-AP 1:600) with HeLa cells lysate 1600 ug.

  • IF Staining of HeLa using 55178-1-AP

    IF Staining of HeLa using 55178-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC2 antibody (55178-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Featured Product
  • KD/KO验证

THOC2 Polyclonal antibody

THOC2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 55178-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

Tho2, THO complex subunit 2, THO complex 2, hTREX120, CXorf3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55178-1-AP targets THOC2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 THO complex 2
别名 Tho2, THO complex subunit 2, THO complex 2, hTREX120, CXorf3
计算分子量 183 kDa
观测分子量 150 kDa
GenBank蛋白编号NM_020449
基因名称 THOC2
Gene ID (NCBI) 57187
RRIDAB_2881283
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NI27
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

THOC2 also termed as THO complex 2 or CXorf3 is 1593 amino acid protein, which belongs to the THOC2 family. THOC2 is a component of heteromultimeric THO/TREX (transcription/export) complex, which is a multi-protein structure consisting of THOC1, THOC2, THOC3, BAT1 and ALY. The TREX complex associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism.

实验方案

Product Specific Protocols
WB protocol for THOC2 antibody 55178-1-APDownload protocol
IF protocol for THOC2 antibody 55178-1-APDownload protocol
IP protocol for THOC2 antibody 55178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Clinical and functional characterization of recurrent missense variants implicated in THOC6-related intellectual disability.

Authors - Mattioli Francesca
View Article
humanWB

Viruses

Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.

Authors - Toshie Sakuma
  • KD Validated
View Article