验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, C2C12 cells, NIH/3T3 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy THAP11 Ready-To-Use IHC Kit. THAP11 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23030-1-AP targets THAP11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag19342 Product name: Recombinant human THAP11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC012182 Sequence: MPGFTCCVPGCYNNSHRDKALHFYTFPKDAELRRLWLKNVSRAGVSGCFSTFQPTTGHRLCSVHFQGGRKTYTVRVPTIFPLRGVNERKVARRPAGAAAARRRQQQQQQQQQQQQQQQQQQQQQQQQQQQQSSPSASTAQTAQLQPNLVSASAAVLLTLQATVDSSQAPGSVQPAPITPTGEDVKPIDLTVQVEFAAAEGAAAAAAASELQAATAGLEAAECPMGPQLVVVGEEGFPDTGSDHSYSLSSGTTEEELLRKLNEQRDILALMEVKMKEMKGSIRHLRLTEAKLREELREKDRLLAMAVIRKKHGM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | THAP domain containing 11 |
| 别名 | CTG B43a, CTG B45d, HRIHFB2206, RONIN, THAP domain-containing protein 11 |
| 计算分子量 | 314 aa, 34 kDa |
| 观测分子量 | 40-50 kDa |
| GenBank蛋白编号 | BC012182 |
| 基因名称 | THAP11 |
| Gene ID (NCBI) | 57215 |
| RRID | AB_2879200 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q96EK4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for THAP11 antibody 23030-1-AP | Download protocol |
| WB protocol for THAP11 antibody 23030-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Res Int THAP11 Functions as a Tumor Suppressor in Gastric Cancer through Regulating c-Myc Signaling Pathways.
| ||
iScience Seizure enhances SUMOylation and zinc-finger transcriptional repression in neuronal nuclei | ||
Adv Sci (Weinh) RONIN/HCF1-TFEB Axis Protects Against D-Galactose-Induced Cochlear Hair Cell Senescence Through Autophagy Activation | ||
J Clin Invest Mutant THAP11 causes cerebellar neurodegeneration and triggers TREM2-mediated microglial activation in mice |
