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验证数据展示

  • WB analysis using 28417-1-AP

    WB analysis using 28417-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28417-1-AP (TH1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 28417-1-AP

    IF Staining of HeLa using 28417-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28417-1-AP (TH1L antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

TH1L Polyclonal antibody

TH1L Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28417-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

HSPC130, Negative elongation factor C/D, NELF C, NELF C/D, NELF D, NELFD, TH1, TH1 like (Drosophila), TH1 like protein, TH1L

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NELFCD/TH1L Ready-To-Use IHC Kit. NELFCD/TH1L primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28417-1-AP targets TH1L in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag29262

Product name: Recombinant human TH1L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 9-150 aa of BC014952

Sequence: IMDEDYYGSAAEWGDEADGGQQEDDSGEGEDDAEVQQECLHKFSTRDYIMEPSIFNTLKRYFQAGGSPENVIQLLSENYTAVAQTVNLLAEWLIQTGVEPVQVQETVENHLKSLLIKHFDPRKADSIFTEEGETPAWLEQMI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TH1-like (Drosophila)
别名 HSPC130, Negative elongation factor C/D, NELF C, NELF C/D, NELF D, NELFD, TH1, TH1 like (Drosophila), TH1 like protein, TH1L
计算分子量 66 kDa
观测分子量 60 kDa
GenBank蛋白编号BC014952
基因名称 TH1L
Gene ID (NCBI) 51497
RRIDAB_2881137
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IXH7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for TH1L antibody 28417-1-APDownload protocol
WB protocol for TH1L antibody 28417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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