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验证数据展示

  • WB analysis of PC-12 using 85929-5-RR (same clone as 85929-5-PBS)

    WB analysis of PC-12 using 85929-5-RR (same clone as 85929-5-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 85929-5-RR (TH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.

  • WB analysis using 85929-5-RR (same clone as 85929-5-PBS)

    WB analysis using 85929-5-RR (same clone as 85929-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85929-5-RR (TH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 85929-5-RR (same clone as 85929-5-PBS)

    IHC staining of mouse brain using 85929-5-RR (same clone as 85929-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85929-5-RR (TH antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85929-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TH antibody included
  • Affinity and Kinetic Characterization of 85929-5-RR

    Affinity and Kinetic Characterization of 85929-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85929-5-RR against Human TH were performed. The affinity constant is 47.4 pM.

TH Recombinant monoclonal antibody, PBS Only

TH Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85929-5-PBS

产品说明书

CloneNo. 250146D7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TH Ready-To-Use IHC Kit. TH primary antibody included.

产品信息

85929-5-PBS targets TH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag23034

Product name: Recombinant human TH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC104967

Sequence: MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAMLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLER

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tyrosine hydroxylase
别名 tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH
计算分子量 528 aa, 59 kDa
观测分子量60 kDa
GenBank蛋白编号BC104967
基因名称 TH
Gene ID (NCBI) 7054
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07101
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons.

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