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验证数据展示

  • WB analysis using 25859-1-AP (same clone as 25859-1-PBS)

    WB analysis using 25859-1-AP (same clone as 25859-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25859-1-AP (TH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    IP experiment of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    IP result of anti-TH (IP:25859-1-AP, 4ug; Detection:25859-1-AP 1:20000) with mouse brain tissue lysate 2120 ug. This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    IHC staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TH antibody included
  • IHC staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    IHC staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TH antibody included
  • IF Staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    IF Staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    IF Staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TH antibody (25859-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    IHC staining of mouse brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25859-1-AP (TH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TH antibody included
  • IF Staining of rat brain using 25859-1-AP (same clone as 25859-1-PBS)

    IF Staining of rat brain using 25859-1-AP (same clone as 25859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded rat brain tissue using TH antibody (25859-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), MAP2 antibody (67015-1-Ig, Clone: 1C3E6, red). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-12 using 25859-1-AP (same clone as 25859-1-PBS)

    IF Staining of PC-12 using 25859-1-AP (same clone as 25859-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using TH antibody (25859-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 25859-1-PBS in a different storage buffer formulation.

TH Polyclonal antibody, PBS Only

TH Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA
Cat No. 25859-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA

别名

tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy TH Ready-To-Use IHC Kit. TH primary antibody included.

产品信息

25859-1-PBS targets TH in WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IF-Fro, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag23075

Product name: Recombinant human TH protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-260 aa of BC104967

Sequence: MPTPDATTPQAKGFRRAVSELDAKQAEAIMSPRFIGRRQSLIEDARKEREAAVAAAAAAVPSEPGDPLEAVAFEEKEGKAMLNLLFSPRATKPSALSRAVKVFETFEAKIHHLETRPAQRPRAGGPHLEYFVRLEVRRGDLAALLSGVRQVSEDVRSPAGPKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQVYRQRRKLIAEIAFQYRHGDPIPRVEYTAEEIATWKEVYTTLKGLYATHACGEHLEAFALLER

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosine hydroxylase
别名 tyrosine hydroxylase, DYT14, DYT5b, EC:1.14.16.2, TYH
计算分子量 528 aa, 59 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC104967
基因名称 TH
Gene ID (NCBI) 7054
RRIDAB_2716568
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07101
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.

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