TGN46 Polyclonal antibody
TGN46 Polyclonal Antibody for IF/ICC,IF-P, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IF/ICC,IF-P, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, HepG2 cells |
Positive IP detected in | HeLa cells |
Positive IF detected in | HeLa cells, A549 cells, human cerebellum tissue, Fibroblast (primary cells) |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13573-1-AP targets TGN46 in WB, IP, IF, ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC,IF-P, IP, WB, ELISA |
Cited Applications | IF, WB |
Tested Reactivity | human |
Cited Reactivity | human, mouse, hamster |
Immunogen | TGN46 fusion protein Ag4470 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | trans-golgi network protein 2 |
Synonyms | Golgi marker, Tgn38, TGN38 homolog, TGN46, TGN48, TGN51, TGOLN2, trans golgi network protein 2, TTGN2 |
Calculated Molecular Weight | 447 aa, 47 kDa |
Observed Molecular Weight | 90-100 kDa |
GenBank Accession Number | BC028219 |
Gene Symbol | TGOLN2 |
Gene ID (NCBI) | 10618 |
RRID | AB_10597396 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O43493 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TGN46 antibody 13573-1-AP | Download protocol |
IF protocol for TGN46 antibody 13573-1-AP | Download protocol |
IP protocol for TGN46 antibody 13573-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Autophagy Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor. | ||
Cell Death Differ Oleate-induced aggregation of LC3 at the trans-Golgi network is linked to a protein trafficking blockade. | ||
PLoS Pathog Host RAB11FIP5 protein inhibits the release of Kaposi's sarcoma-associated herpesvirus particles by promoting lysosomal degradation of ORF45. | ||
Int J Mol Sci Novel Insights into Selected Disease-Causing Mutations within the SLC35A1 Gene Encoding the CMP-Sialic Acid Transporter. |