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验证数据展示

  • WB analysis using RMX00075

    WB analysis using RMX00075

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00075 (TGN46 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using RMX00075

    IHC staining of human liver using RMX00075

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using RMX00075 (TGN46 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using RMX00075

    IHC staining of human liver using RMX00075

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using RMX00075 (TGN46 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using RMX00075

    IF Staining of HeLa using RMX00075

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TGN46 antibody (RMX00075) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Multi-rAb® TGN46 Multi-Recombinant antibody

TGN46 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. RMX00075

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

TGOLN2, hTGN46, hTGN48, hTGN51, TGN38

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00075 targets TGN46 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 trans-golgi network protein 2
别名 TGOLN2, hTGN46, hTGN48, hTGN51, TGN38
计算分子量 447 aa, 47 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC028219
基因名称 TGN46
Gene ID (NCBI) 10618
RRIDAB_3750124
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDO43493
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TGN46 (TGOLN2), the human homolog of rat Tgn38, is a transmembrane glycoprotein predominantly localized to the TGN (trans-Golgi network). TGN is a major secretory pathway sorting station for proteins and lipids. TGN46 may be involved in regulating membrane traffic to and from TGN. Alternatively, spliced transcript variants encode different TGN46 isoforms. TGN46 has an apparent molecular mass of 100-150 kDa, suggesting extensive O- and N-glycosylations.

实验方案

Product Specific Protocols
IF protocol for TGN46 antibody RMX00075Download protocol
IHC protocol for TGN46 antibody RMX00075Download protocol
WB protocol for TGN46 antibody RMX00075Download protocol
Standard Protocols
Click here to view our Standard Protocols
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