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验证数据展示

  • WB analysis of mouse testis using 15847-1-AP

    WB analysis of mouse testis using 15847-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 15847-1-AP

    WB analysis using 15847-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15847-1-AP (TGM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 15847-1-AP

    IHC staining of human testis using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 15847-1-AP

    IHC staining of human testis using 15847-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 15847-1-AP (TGM4 antibody) at dilution of 1:100 (under 40x lens).

TGM4 Polyclonal antibody

TGM4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15847-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Fibrinoligase, hTGP, Prostate transglutaminase, TG(P), TGase 4, TGase P, TGM4, TGP, Transglutaminase 4, transglutaminase 4 (prostate), Transglutaminase P

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, human testis tissue, HeLa cells, PC-3 cells, rat testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15847-1-AP targets TGM4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8608

Product name: Recombinant human TGM4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 335-684 aa of BC007003

Sequence: KRPDLPKGYDGWQAVDATSQERSQGVFCCGPSPLTAIRKGDIFIVYDTRFVFSEVNGDRLIWLVKMVNGQEELHVISMETTSIGKNISTKAVGQDRRRDITYEYKYPEGSSEERQVMDHAFLLLSSEREHRRPVKENFLHMSVQSDDVLLGNSVNFTVILKRKTAALQNVNILGSFELQLYTGKKMAKLCDLNKTSQIQGQVSEVTLTLDSKTYINSLAILDDEPVIRGFIIAEIVESKEIMASEVFTSFQYPEFSIELPNTGRIGQLLVCNCIFKNTLAIPLTDVKFSLESLGISSLQTSDHGTVQPGETIQSQIKCTPIKTGPKKFIVKLSSKQVKEINAQKIVLITK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transglutaminase 4 (prostate)
别名 Fibrinoligase, hTGP, Prostate transglutaminase, TG(P), TGase 4, TGase P, TGM4, TGP, Transglutaminase 4, transglutaminase 4 (prostate), Transglutaminase P
计算分子量 684 aa, 77 kDa
观测分子量 68-70 kDa
GenBank蛋白编号BC007003
基因名称 TGM4
Gene ID (NCBI) 7047
RRIDAB_2287328
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49221
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TGM4 antibody 15847-1-APDownload protocol
IHC protocol for TGM4 antibody 15847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols