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验证数据展示

  • WB analysis using 84032-2-RR

    WB analysis using 84032-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84032-2-RR (TGM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84032-2-RR

    WB analysis using 84032-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84032-2-RR (TGM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human bowen disease using 84032-2-RR

    IHC staining of human bowen disease using 84032-2-RR

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 84032-2-RR (TGM1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TGM1 Recombinant antibody

TGM1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84032-2-RR

产品说明书

CloneNo. 241218E12

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

LI, KTG, ICR2, Epidermal TGase, EC:2.3.2.13

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman saliva, rat liver tissue
Positive IHC detected inhuman bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84032-2-RR targets TGM1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 TGM1 fusion protein Ag17969 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)
别名 LI, KTG, ICR2, Epidermal TGase, EC:2.3.2.13
计算分子量 817 aa, 90 kDa
观测分子量90 kDa
GenBank蛋白编号BC034699
基因名称 TGM1
Gene ID (NCBI) 7051
RRIDAB_3671599
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP22735
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TGM1 antibody 84032-2-RRDownload protocol
IHC protocol for TGM1 antibody 84032-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols