TGIF2 Polyclonal antibody
TGIF2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, Daudi cells, HL-60 cells, Jurkat cells |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Caco-2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
产品信息
11522-1-AP targets TGIF2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | TGIF2 fusion protein Ag2085 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | TGFB-induced factor homeobox 2 |
Synonyms | 5 TG 3 interacting factor 2, Homeobox protein TGIF2, TGFB induced factor 2, TGFB induced factor homeobox 2, TGIF2 |
Calculated Molecular Weight | 237 aa, 26 kDa |
Observed Molecular Weight | 28 kDa and 30 kDa |
GenBank Accession Number | BC012816 |
Gene Symbol | TGIF2 |
Gene ID (NCBI) | 60436 |
RRID | AB_2202731 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9GZN2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TGIF2 antibody 11522-1-AP | Download protocol |
IHC protocol for TGIF2 antibody 11522-1-AP | Download protocol |
IF protocol for TGIF2 antibody 11522-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Rep Tgif1 Counterbalances the Activity of Core Pluripotency Factors in Mouse Embryonic Stem Cells. | ||
Biochem J The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome. | ||
Exp Ther Med Molecular mechanisms in vascular injury induced by hypertension: Expression and role of microRNA-34a. | ||
Brain Behav Downregulation of TGIF2 is possibly correlated with neuronal apoptosis and autism-like symptoms in mice. |