验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, MCF-7 cells, COLO320 cells | 
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 | 
推荐稀释比
| 应用 | 推荐稀释比 | 
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 | 
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 10 publications below | 
| IHC | See 1 publications below | 
产品信息
27212-1-AP targets TGFBR2 in WB, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description | 
| 文献引用应用 | WB, IHC | 
| 经测试反应性 | human | 
| 文献引用反应性 | human, mouse, rat, sheep | 
| 免疫原 | CatNo: Ag25767 Product name: Recombinant human TGFBR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-166 aa of BC040499 Sequence: TIPPHVQKSVNNDMIVTDNNGAVKFPQLCKFCDVRFSTCDNQKSCMSNCSITSICEKPQEVCVAVWRKNDENITLETVCHDPKLPYHDFILEDAASPKCIMKEKKKPGETFFMCSCSSDECNDNIIFSEEYNTSNPDLLLVIFQ种属同源性预测 | 
| 宿主/亚型 | Rabbit / IgG | 
| 抗体类别 | Polyclonal | 
| 产品类型 | Antibody | 
| 全称 | transforming growth factor, beta receptor II (70/80kDa) | 
| 别名 | EC:2.7.11.30, TbetaR-II, TGF beta receptor type 2, TGF-beta receptor type II, TGF-beta receptor type-2 | 
| 计算分子量 | 65 kDa | 
| 观测分子量 | 65-80 kDa | 
| GenBank蛋白编号 | BC040499 | 
| 基因名称 | TGFBR2 | 
| Gene ID (NCBI) | 7048 | 
| RRID | AB_2918119 | 
| 偶联类型 | Unconjugated | 
| 形式 | Liquid | 
| 纯化方式 | Antigen affinity purification | 
| UNIPROT ID | P37173 | 
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. | 
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
背景介绍
TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). The calculated molecular weight of TGFBR2 is 65 kDa. It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TGFBR2 antibody 27212-1-AP | Download protocol | 
| WB protocol for TGFBR2 antibody 27212-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 
发表文章
| Species | Application | Title | 
|---|---|---|
| Biochim Biophys Acta Mol Cell Res YWK-II/APLP2 inhibits TGF-β signaling by interfering with the TGFBR2-Hsp90 interaction | ||
| Cell Commun Signal The activation of PPARγ enhances Treg responses through up-regulating CD36/CPT1-mediated fatty acid oxidation and subsequent N-glycan branching of TβRII/IL-2Rα. | ||
| Reprod Sci Maternal Perinatal Exposure to Dibutyl Phthalate Promotes Ovarian Dysfunction in Adult Female Offspring via Downregulation of TGF-β2 and TGF-β3. | ||
| Cell Chem Biol Therapeutic potency of compound RMY-205 for pulmonary fibrosis induced by SARS-CoV-2 nucleocapsid protein | ||
| Acta Histochem Inhibition of glutamine transporter ASCT2 mitigates bleomycin-induced pulmonary fibrosis in mice | 



