验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | A549 cells, U-87 MG cells |
Planning an IHC experiment? We recommend our IHCeasy TGFB2 Ready-To-Use IHC Kit. TGFB2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:125-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83167-5-RR targets TGFB2/TGF-beta 2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | transforming growth factor, beta 2 |
| 别名 | TGFB2-Specific, TGFB2, TGF-beta 2, 230461F5, Glioblastoma-derived T-cell suppressor factor |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | NM_003238 |
| 基因名称 | TGFB2 |
| Gene ID (NCBI) | 7042 |
| RRID | AB_3670862 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P61812 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Tranforming growth factor beta-2 (TGFB2), also named G-TSF, BSC-1 cell growth inhibitor, Polyergin and Cetermin, belongs to the TGF-beta family. TGFB2 is a secreted protein and contains a 19aa signal peptides at its N-terminal. Heterodimers with TGFB1 and with TGFB2 have been found in bone. It was proved to have suppressive effects on interleukin-2 dependent T-cell growth.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for TGFB2/TGF-beta 2 antibody 83167-5-RR | Download protocol |
| IF protocol for TGFB2/TGF-beta 2 antibody 83167-5-RR | Download protocol |
| IHC protocol for TGFB2/TGF-beta 2 antibody 83167-5-RR | Download protocol |
| WB protocol for TGFB2/TGF-beta 2 antibody 83167-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
