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TGF Alpha Polyclonal antibody

TGF Alpha Polyclonal Antibody for IF/ICC, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 22308-1-AP

Print datasheet

Synonyms

EGF like TGF, ETGF, TFGA, TGF alpha, TGF type 1, TGFA

验证数据展示

  • IHC staining of human breast cancer using 22308-1-AP

    IHC staining of human breast cancer using 22308-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22308-1-AP (TGF-alpha antibody at dilution of 1:50.

  • IHC staining of human breast cancer using 22308-1-AP

    IHC staining of human breast cancer using 22308-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22308-1-AP (TGF-alpha antibody at dilution of 1:50.

  • IF Staining of A431 using 22308-1-AP

    IF Staining of A431 using 22308-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 22308-1-AP (TGF-alpha antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman breast cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 2 publications below

产品信息

22308-1-AP targets TGF Alpha in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transforming growth factor, alpha
Synonyms EGF like TGF, ETGF, TFGA, TGF alpha, TGF type 1, TGFA
Calculated Molecular Weight 17 kDa
GenBank Accession NumberNM_003236
Gene Symbol TGFA
Gene ID (NCBI) 7039
RRIDAB_2879069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01135
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TGF Alpha antibody 22308-1-APDownload protocol
IF protocol for TGF Alpha antibody 22308-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

J Cell Mol Med

CircRhoC promotes tumorigenicity and progression in ovarian cancer by functioning as a miR-302e sponge to positively regulate VEGFA.

Authors - Li-Li Wang
View Article
humanIHC

Mol Cancer

MicroRNA-505 functions as a tumor suppressor in endometrial cancer by targeting TGF-α.

Authors - Shuo Chen
View Article
human

Drug Des Devel Ther

Adenosine Inhibits Ovarian Cancer Growth Through Regulating RhoGDI2 Protein Expression.

Authors - Bing Xia
View Article
humanIHC

Int J Exp Pathol

Clinicopathological features and immunophenotype of Silva pattern system in endocervical adenocarcinoma

Authors - Chao Zeng
View Article