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验证数据展示

  • WB analysis of K-562 using 10097-2-AP (same clone as 10097-2-PBS)

    WB analysis of K-562 using 10097-2-AP (same clone as 10097-2-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 10097-2-AP (TFPT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10097-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 10097-2-AP (same clone as 10097-2-PBS)

    IHC staining of mouse liver using 10097-2-AP (same clone as 10097-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10097-2-AP (TFPT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10097-2-PBS in a different storage buffer formulation.

TFPT Polyclonal antibody, PBS Only

TFPT Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10097-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

TCF3 fusion partner, Protein FB1, INO80F, INO80 complex subunit F, FB1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10097-2-PBS targets TFPT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0131

Product name: Recombinant human TFPT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 39-253 aa of BC001728

Sequence: SELETEVEFVSGGLGGSGLRERDEEEEAARGRRRRQRELNRRKYQALGRRCREIEQVNERVLNRLHQVQRITRRLQQERRFLMRVLDSYGDDYRASQFTIVLEDEGSQGTDAPTPGNAENEPPEKETLSPPRRTPAPPEPGSPAPGEGPSGRKRRRVPRDGRRAGNALTPELAPVQIKVEEDFGFEADEALDSSWVSRGPDKLLPYPTLASPAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TCF3 (E2A) fusion partner (in childhood Leukemia)
别名 TCF3 fusion partner, Protein FB1, INO80F, INO80 complex subunit F, FB1
计算分子量 29 kDa
观测分子量 29 kDa
GenBank蛋白编号BC001728
基因名称 TFPT
Gene ID (NCBI) 29844
RRIDAB_2240572
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP0C1Z6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TFPT/FB1 is one of three fusion partners of the E2A gene. TFPT/FB1 is expressed at low level in a variety of human tissues, including hemopoietic cell lines from different lineages. The function of the putative TFPT/FB1 protein, however, is currently unknown as database sequence comparisons have failed to reveal strong homology with known proteins. The E2A-TFPT/FB1 fusion appears to be a recurrent feature of pre-B ALLs, suggesting that it might have a role in the development and/or progression of leukemogenesis. The gene encoding TFPT/FB1 is localized on 19q13.4.

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