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TFIP11 Polyclonal antibody

TFIP11 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14436-1-AP

Print datasheet

Synonyms

bK445C9.6, STIP, STIP 1, TFIP11, TIP39

验证数据展示

  • WB analysis of HeLa using 14436-1-AP

    WB analysis of HeLa using 14436-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14436-1-AP (TFIP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14436-1-AP

    IF Staining of HeLa using 14436-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14436-1-AP (TFIP11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, mouse testis tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14436-1-AP targets TFIP11 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen TFIP11 fusion protein Ag5821 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tuftelin interacting protein 11
Synonyms bK445C9.6, STIP, STIP 1, TFIP11, TIP39
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC033080
Gene Symbol TFIP11
Gene ID (NCBI) 24144
RRIDAB_11182269
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBB9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.

实验方案

Product Specific Protocols
WB protocol for TFIP11 antibody 14436-1-APDownload protocol
IF protocol for TFIP11 antibody 14436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols