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验证数据展示

  • WB analysis of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

    WB analysis of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

    human stomach tissue were subjected to SDS PAGE followed by western blot with 13681-1-AP (TFF2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

    IHC staining of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13681-1-AP (TFF2 Antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

    IHC staining of human stomach using 13681-1-AP (same clone as 13681-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13681-1-AP (TFF2 Antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

    IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

    IF Staining of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse stomach tissue using TFF2 antibody (13681-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

    IP experiment of mouse stomach using 13681-1-AP (same clone as 13681-1-PBS)

    IP result of anti-TFF2 (IP:13681-1-AP, 4ug; Detection:13681-1-AP 1:600) with mouse stomach tissue lysate 4000ug. This data was developed using the same antibody clone with 13681-1-PBS in a different storage buffer formulation.

TFF2 Polyclonal antibody, PBS Only

TFF2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA
Cat No. 13681-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, Indirect ELISA

别名

SML1, SP, Spasmolysin, Spasmolytic polypeptide, trefoil factor 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13681-1-PBS targets TFF2 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4537

Product name: Recombinant human TFF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-129 aa of BC032820

Sequence: MGRRDAQLLAALLVLGLCALAGSEKPSPCQCSRLSPHNRTNCGFPGITSDQCFDNGCCFDSSVTGVPWCFHPLPKQESDQCVMEVSDRRNCGYPGISPEECASRKCCFSNFIFEVPWCFFPKSVEDCHY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trefoil factor 2
别名 SML1, SP, Spasmolysin, Spasmolytic polypeptide, trefoil factor 2
计算分子量 129 aa, 14 kDa
观测分子量 18-20 kDa
GenBank蛋白编号BC032820
基因名称 TFF2
Gene ID (NCBI) 7032
RRIDAB_10598321
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03403
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The trefoil factor family (TFF), comprises of three polypeptides, TFF1, TFF2 and TFF3 (7-12 kDa), secreted to mucosal surfaces by mucus producing cells, prominently in the gastrointestinal tract. TFF2, also known as spasmolytic polypeptide, is a low-molecular weight protein, expressed in mucous neck cells of the fundus and glands at the base of the antrum in normal human stomach. TFF2 could Inhibit gastrointestinal motility and gastric acid secretion. However, recent studies suggest that TFF2 could also play an important role in the immune system. We got a 18-20 kDa band in western botting maybe due to glycosylatation, and mature TFF2 is a 12 kDa protein (PMID: 10716671).

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