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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84675-5-RR (same clone as 84675-5-PBS)

    WB analysis using 84675-5-RR (same clone as 84675-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84675-5-RR (TFEB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84675-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84675-5-RR (same clone as 84675-5-PBS)

    IF Staining of HeLa using 84675-5-RR (same clone as 84675-5-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TFEB antibody (84675-5-RR, Clone: 242093D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84675-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84675-5-RR

    Affinity and Kinetic Characterization of 84675-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84675-5-RR against Human TFEB were performed. The affinity constant is 7.61 nM.

    {{ptg:ProductFeaturesInfo}}

TFEB Recombinant monoclonal antibody, PBS Only

TFEB Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84675-5-PBS

产品说明书

CloneNo. 242093D11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

242093D11, Alpha TFEB, AlphaTFEB, bHLHe35, Class E basic helix-loop-helix protein 35

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TFEB Ready-To-Use IHC Kit. TFEB primary antibody included.

产品信息

84675-5-PBS targets TFEB in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4194

Product name: Recombinant human TFEB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-476 aa of BC032448

Sequence: MASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDNIMRLDDVLGYINPEMQMPNTLPLSSSHLNVYSSDPQVTASLVGVTSSSCPADLTQKRELTDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGMNMAELAQQVVKQELPSEEGPGEALMLGAEVPDPEPLPALPPQAPLPLPTQPPSPFHHLDFSHSLSFGGREDEGPPGYPEPLAPGHGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMSPEASKASSRRSSFSMEEGDVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcription factor EB
别名 242093D11, Alpha TFEB, AlphaTFEB, bHLHe35, Class E basic helix-loop-helix protein 35
计算分子量 476 aa, 53 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC032448
基因名称 TFEB
Gene ID (NCBI) 7942
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP19484
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).