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TFEB Polyclonal antibody

TFEB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 13372-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

Alpha TFEB, AlphaTFEB, bHLHe35, Class E basic helix-loop-helix protein 35, TCFEB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, Jurkat cells, HeLa cells, human placenta tissue, NIH/3T3 cells, rat brain tissue, HEK-293 cells, Raji cells, C2C12 cells, RAW 264.7 cells, mouse heart tissue, rat heart tissue
Positive IP detected inRaji cells
Positive IHC detected inhuman renal cell carcinoma tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TFEB Ready-To-Use IHC Kit. TFEB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13372-1-AP targets TFEB in WB, IHC, IF/ICC, IP, CoIP, ELISA, Wes Immunoassay applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, Wes Immunoassay
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, monkey, zebrafish, bovine
免疫原 TFEB fusion protein Ag4194 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription factor EB
别名 Alpha TFEB, AlphaTFEB, bHLHe35, Class E basic helix-loop-helix protein 35, TCFEB
计算分子量 476 aa, 53 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC032448
基因名称 TFEB
Gene ID (NCBI) 7942
RRIDAB_2199611
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP19484
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB. The calculated molecular weight of TFEB is 53 kDa, but modified TFEB is about 65-70 kDa (PMID: 12837690). TFEB mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). Fisetin increases the levels of TFEB localized in nuclei (PMID: 27112200).

实验方案

Product Specific Protocols
WB protocol for TFEB antibody 13372-1-APDownload protocol
IHC protocol for TFEB antibody 13372-1-APDownload protocol
IF protocol for TFEB antibody 13372-1-APDownload protocol
IP protocol for TFEB antibody 13372-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

ACS Nano

A Self-Assembled α-Synuclein Nanoscavenger for Parkinson's Disease.

Authors - Jingyi Liu
mouseIF

Acta Neuropathol

Wolframin is a novel regulator of tau pathology and neurodegeneration.

Authors - Shuo Chen
mouseWB,IP,IF

Mol Cell

Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.

Authors - Zhenxing Zhang
mouseWB,IF

J Hazard Mater

VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes

Authors - Tao Wang
humanWB,IF

Autophagy

SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis

Authors - Qin Xia
humanWB

Cell Death Differ

Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration

Authors - Huaizhen Liang
  • KD Validated
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