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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 67319-1-Ig

    WB analysis using 67319-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67319-1-Ig (TFE3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human malignant melanoma using 67319-1-Ig

    IHC staining of human malignant melanoma using 67319-1-Ig

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 67319-1-Ig (TFE3 antibody) at dilution of 1:200 (under 40x lens)..

    Ready-to-use IHC kits available with TFE3 antibody included

TFE3 Monoclonal antibody

TFE3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67319-1-Ig

产品说明书

CloneNo. 1E3D7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

别名

1E3D7, bHLHe33, Class E basic helix-loop-helix protein 33, RCCP2, TFE 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, C2C12 cells, pig spleen tissue, HeLa cells, Raji cells, K-562 cells, rat spleen tissue
Positive IHC detected inhuman malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TFE3 Ready-To-Use IHC Kit. TFE3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67319-1-Ig targets TFE3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag6320

Product name: Recombinant human TFE3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC026027

Sequence: MSHAAEPARDGVEASAEGPRAVFVLLEERRPADSAQLLSLNSLLPESGIVADIELENVLDPDSFYELKSQPLPLRSSLPISLQATPATPATLSASSSAGGSRTPAMSSSSSSRVLLRQQLMRAQAQEQERRERREQAAAAPFPSPAPASPAISVVGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPKLASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDN

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transcription factor binding to IGHM enhancer 3
别名 1E3D7, bHLHe33, Class E basic helix-loop-helix protein 33, RCCP2, TFE 3
计算分子量 62 kDa
观测分子量 70 kDa
GenBank蛋白编号BC026027
基因名称 TFE3
Gene ID (NCBI) 7030
RRIDAB_2882579
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP19532
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TFE3 antibody 67319-1-IgDownload protocol
IHC protocol for TFE3 antibody 67319-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols