验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, human brain tissue |
| Positive IP detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
11500-1-AP targets TFDP2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2054 Product name: Recombinant human TFDP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 38-387 aa of BC021113 Sequence: GWVPGDRKRARKFIDSDFSESKRSKKGDKNGKGLRHFSMKVCEKVQRKGTTSYNEVADELVSEFTNSNNHLAADSQAYDQKNIRRRVYDALNVLMAMNIISKEKKEIKWIGLPTNSAQECQNLEIEKQRRIERIKQKRAQLQELLLQQIAFKNLVQRNRQNEQQNQGPPALNSTIQLPFIIINTSRKTVIDCSISSDKFEYLFNFDNTFEIHDDIEVLKRMGMSFGLESGKCSLEDLKLAKSLVPKALEGYITDISTGPSWLNQGLLLNSTQSVSNLDLTTGATLPQSSVNQGLCLDAEVALATGQFLAPNSHQSSSAASHCSESRGETPCSFNDEDEEDDEEDSSSPE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcription factor Dp-2 (E2F dimerization partner 2) |
| 别名 | Transcription factor Dp-2, DP2, Dp 2 |
| 计算分子量 | 446 aa, 49 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC021113 |
| 基因名称 | TFDP2 |
| Gene ID (NCBI) | 7029 |
| RRID | AB_2202685 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14188 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TFDP2, also known as E2FDP2, is a coactivator of E2F factors and involves in EGF or IFN-gamma-mediated effects by enhancing or reducing active heterodimeric complexes. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis [PMID:16360038]. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication [PMID:16721044].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TFDP2 antibody 11500-1-AP | Download protocol |
| IP protocol for TFDP2 antibody 11500-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Syst Biol The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation. | ||
Front Mol Biosci Comprehensive analysis of senescence-related genes and immune infiltration in intervertebral disc degeneration: a meta-data approach utilizing bulk and single-cell RNA sequencing data | ||
Neuropharmacology The Novel miR_146-Tfdp2 Axis Antagonizes METH-Induced Neuron Apoptosis and Cell Cycle Abnormalities in Tree Shrew |
