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TFDP2 Polyclonal antibody

TFDP2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11500-1-AP

Print datasheet

Synonyms

Dp 2, DP2, E2F dimerization partner 2, TFDP2, Transcription factor Dp 2

验证数据展示

  • WB analysis of mouse heart using 11500-1-AP

    WB analysis of mouse heart using 11500-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 11500-1-AP

    WB analysis of human brain using 11500-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11500-1-AP (TFDP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of SH-SY5Y using 11500-1-AP

    IP experiment of SH-SY5Y using 11500-1-AP

    IP result of anti-TFDP2 (IP:11500-1-AP, 4ug; Detection:11500-1-AP 1:400) with SH-SY5Y cells lysate 1600ug.

经过测试的应用

Positive WB detected inmouse heart tissue, human brain tissue
Positive IP detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11500-1-AP targets TFDP2 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen TFDP2 fusion protein Ag2054 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription factor Dp-2 (E2F dimerization partner 2)
Synonyms Dp 2, DP2, E2F dimerization partner 2, TFDP2, Transcription factor Dp 2
Calculated Molecular Weight 446 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC021113
Gene Symbol TFDP2
Gene ID (NCBI) 7029
RRIDAB_2202685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14188
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TFDP2, also known as E2FDP2, is a coactivator of E2F factors and involves in EGF or IFN-gamma-mediated effects by enhancing or reducing active heterodimeric complexes. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis [PMID:16360038]. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication [PMID:16721044].

实验方案

Product Specific Protocols
WB protocol for TFDP2 antibody 11500-1-APDownload protocol
IP protocol for TFDP2 antibody 11500-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Syst Biol

The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation.

Authors - Caron Etienne E
View Article