验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue, rat spleen tissue, rat brain tissue, mouse kidney tissue, K-562 cells |
| Positive IP detected in | mouse spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15203-1-AP targets TFCP2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag7353 Product name: Recombinant human TFCP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-348 aa of BC003634 Sequence: MAWALKLPLADEVIESGLVQDFDASLSGIGQELGAGAYSMSDVLALPIFKQEESSLPPDNENKILPFQYVLCAATSPAVKLHDETLTYLNQGQSYEIRMLDNRKLGELPEINGKLVKSIFRVVFHDRRLQYTEHQQLEGWRWNRPGDRILDIDIPMSVGIIDPRANPTQLNTVEFLWDPAKRTSVFIQVHCISTEFTMRKHGGEKGVPFRVQIDTFKENENGEYTEHLHSASCQIKVFKPKGADRKQKTDREKMEKRTPHEKEKYQPSYETTILTECSPWPEITYVNNSPSPGFNSSHSSFSLGEGNGSPNHQPEPPPPVTDNLLPTTTPQEAQQWLHRNRFSTFTRL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcription factor CP2 |
| 别名 | CP2, LBP 1C, LSF, SAA3 enhancer factor, SEF, TFCP2, TFCP2C, transcription factor CP2, Transcription factor LSF |
| 计算分子量 | 57 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC003634 |
| 基因名称 | TFCP2 |
| Gene ID (NCBI) | 7024 |
| RRID | AB_2199582 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q12800 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TFCP2, also named as SAA3 enhancer factor and transcription factor LSF, is a 502 amino acid protein, which belongs to the grh/CP2 family. TFCP2 is expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung. TFCP2 binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. The calculated molecular weight of TFCP2 is 57 kDa Similar levels of TFCP2 migrating at 64-66 kDa were evident in all three samples. (PMID: 7828600)
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for TFCP2 antibody 15203-1-AP | Download protocol |
| WB protocol for TFCP2 antibody 15203-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Environ Pollut Early-life exposure to bisphenol A induces dysregulation of lipid homeostasis by the upregulation of SCD1 in male mice. | ||
Cell Death Dis MicroRNA-144 is regulated by CP2 and decreases COX-2 expression and PGE2 production in mouse ovarian granulosa cells. | ||
Clin Epigenetics DNA hypermethylation contributes to colorectal cancer metastasis by regulating the binding of CEBPB and TFCP2 to the CPEB1 promoter. | ||
Front Oncol TFCP2 Overcomes Senescence by Cooperating With SREBP2 to Activate Cholesterol Synthesis in Pancreatic Cancer.
| ||
Exp Cell Res TFCP2, a binding protein of ATF3, promotes the progression of glioma by activating the synthesis of serine.
| ||
J Exp Clin Cancer Res Overexpressed integrin alpha 2 inhibits the activation of the transforming growth factor β pathway in pancreatic cancer via the TFCP2-SMAD2 axis. |
