验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
产品信息
24411-1-AP targets TFB2M in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, zebrafish |
| 免疫原 |
CatNo: Ag19687 Product name: Recombinant human TFB2M protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 176-396 aa of BC003383 Sequence: LGIEAVPWTADIPLKVVGMFPSRGEKRALWKLAYDLYSCTSIYKFGRIEVNMFIGEKEFQKLMADPGNPDLYHVLSVIWQLACEIKVLHMEPWSSFDIYTRKGPLENPKRRELLDQLQQKLYLIQMIPRQNLFTKNLTPMNYNIFFHLLKHCFGRRSATVIDHLRSLTPLDARDILMQIGKQEDEKVVNMHPQDFKTLFETIERSKDCAYKWLYDETLEDR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcription factor B2, mitochondrial |
| 别名 | h mtTFB, h mtTFB2, Hkp1, hTFB2M, mtTFB2, NS5ATP5, TFB2M |
| 计算分子量 | 45 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC003383 |
| 基因名称 | TFB2M |
| Gene ID (NCBI) | 64216 |
| RRID | AB_2879530 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H5Q4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TFB2M (mitochondrial transcription factor B2) is a novel nuclear-encoded protein that is necessary for transcription of mitochondrial DNA.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TFB2M antibody 24411-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Spliceosomal proofreading factors safeguard 3' splice-site fidelity and prevent proteotoxicity and inflammation | ||
Nat Chem Biol Mitochondria-localized MBD2c facilitates mtDNA transcription and drug resistance | ||
Nucleic Acids Res Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function. | ||
Cell Death Dis Hypoxic tumor exosomes suppress macrophage inflammation and ferroptosis via NDUFV2 to enhance bystander tumor radioresistance. | ||
Cells A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication
| ||
Biochim Biophys Acta Mol Basis Dis LARP1, an RNA-binding protein, participates in ovarian cancer cell survival by regulating mitochondrial oxidative phosphorylation in response to the influence of the PI3K/mTOR pathway |
