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验证数据展示

  • WB analysis using 84957-4-RR (same clone as 84957-4-PBS)

    WB analysis using 84957-4-RR (same clone as 84957-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84957-4-RR (TFB1M antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 84957-4-RR (same clone as 84957-4-PBS)

    IP experiment of HepG2 using 84957-4-RR (same clone as 84957-4-PBS)

    IP result of anti-TFB1M (IP:84957-4-RR, 4ug; Detection:84957-4-RR 1:1500) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84957-4-RR (same clone as 84957-4-PBS)

    IHC staining of human colon cancer using 84957-4-RR (same clone as 84957-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84957-4-RR (TFB1M antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84957-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84957-4-RR

    Affinity and Kinetic Characterization of 84957-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84957-4-RR against Human TFB1M were performed. The affinity constant is 2.99 nM.

TFB1M Recombinant monoclonal antibody, PBS Only

TFB1M Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 84957-4-PBS

产品说明书

CloneNo. 242524A10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

242524A10, CGI 75, CGI75, CGI-75, EC:2.1.1.-

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84957-4-PBS targets TFB1M in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9785

Product name: Recombinant human TFB1M protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-346 aa of BC017788

Sequence: MAASGKLSTCRLPPLPTIREIIKLLRLQAAKQLSQNFLLDLRLTDKIVRKAGNLTNAYVYEVGPGPGGITRSILNADVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVHGDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPLIIKWLENISCRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHIFTIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKYCHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISHFKSLCDVYRKMCDEDPQLFAYNFREELKRRKSKNEEKEEDDAENYRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcription factor B1, mitochondrial
别名 242524A10, CGI 75, CGI75, CGI-75, EC:2.1.1.-
计算分子量 346 aa, 40 kDa
观测分子量40 kDa
GenBank蛋白编号BC017788
基因名称 TFB1M
Gene ID (NCBI) 51106
RRIDAB_3743884
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ8WVM0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitochondrial transcription factor B1 (TFB1M) is a dimethyltransferase that maintains mitochondrial homeostasis by catalyzing dimethylation of two adjacent adenines located in helix45 (h45) of 12S rRNA. Mitochondrial transcription specificity factors (TFB1M and TFB2M), which markedly enhance mtDNA transcription in the presence of Tfam and mitochondrial RNA polymerase, have been identified in mammalian cells. The expression of human TFB1M and TFB2M promoters is governed by nuclear respiratory factors (NRF-1 and NRF-2), key transcription factors implicated in mitochondrial biogenesis. (PMID: 31251801, PMID: 15684387)

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