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验证数据展示

  • WB analysis using 83196-4-RR

    WB analysis using 83196-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83196-4-RR (TFAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 83196-4-RR

    WB analysis of HeLa using 83196-4-RR

    WB result of TFAP4 antibody (83196-4-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TFAP4 transfected HeLa cells.

  • IF Staining of HeLa using 83196-4-RR

    IF Staining of HeLa using 83196-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFAP4 antibody (83196-4-RR, Clone: 240031D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 83196-4-RR

    Affinity and Kinetic Characterization of 83196-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83196-4-RR against Human TFAP4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

TFAP4 Recombinant monoclonal antibody

TFAP4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83196-4-RR

产品说明书

CloneNo. 240031D5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

240031D5, Activating enhancer-binding protein 4, AP 4, bHLHc41, Class C basic helix-loop-helix protein 41

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, A549 cells, NCI-H1299 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83196-4-RR targets TFAP4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2633

Product name: Recombinant human TFAP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-338 aa of BC010576

Sequence: MEYFMVPTQKVPSLQHFRKTEKEVIGGLCSLANIPLTPETQRDQERRIRREIANSNERRRMQSINAGFQSLKTLIPHTDGEKLSKAAILQQTAEYIFSLEQEKTRLLQQNTQLKRFIQELSGSSPKRRRAEDKDEGIGSPDIWEDEKAEDLRREMIELRQQLDKERSVRMMLEEQVRSLEAHMYPEKLKVIAQQVQLQQQQEQVRLLHQEKLEREQQQLRTQLLPPPAPTHHPTVIVPAPPPPPSHHINVVTMGPSSVINSVSTSRQNLDTIVQAIQHIEGTQEKQELEEEQRRAVIVKPVRSCPEAPTSDTASDSEASDSDAMDQSREEPSGDGELP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcription factor AP-4 (activating enhancer binding protein 4)
别名 240031D5, Activating enhancer-binding protein 4, AP 4, bHLHc41, Class C basic helix-loop-helix protein 41
计算分子量 338 aa, 39 kDa
观测分子量39-42 kDa
GenBank蛋白编号BC010576
基因名称 TFAP4
Gene ID (NCBI) 7023
RRIDAB_3670886
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ01664
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for TFAP4 antibody 83196-4-RRDownload protocol
WB protocol for TFAP4 antibody 83196-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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