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TFAP4 Polyclonal antibody

TFAP4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat No : 12017-1-AP

Print datasheet

Synonyms

AP 4, bHLHc41, TFAP4, Transcription factor AP 4

验证数据展示

  • WB analysis using 12017-1-AP

    WB analysis using 12017-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12017-1-AP (TFAP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 12017-1-AP

    WB analysis using 12017-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12017-1-AP (TFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12017-1-AP

    IP experiment of HeLa using 12017-1-AP

    IP result of anti-TFAP4 (IP:12017-1-AP, 4ug; Detection:12017-1-AP 1:1000) with HeLa cells lysate 1360 ug.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, NCI-H1299 cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

12017-1-AP targets TFAP4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TFAP4 fusion protein Ag2633 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription factor AP-4 (activating enhancer binding protein 4)
Synonyms AP 4, bHLHc41, TFAP4, Transcription factor AP 4
Calculated Molecular Weight 338 aa, 39 kDa
Observed Molecular Weight 39-42 kDa
GenBank Accession NumberBC010576
Gene Symbol TFAP4
Gene ID (NCBI) 7023
RRIDAB_2199571
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01664
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TFAP4 antibody 12017-1-APDownload protocol
IP protocol for TFAP4 antibody 12017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Agric Food Chem

Cucurbitacin E Chemosensitizes Colorectal Cancer Cells via Mitigating TFAP4/Wnt/β-Catenin Signaling.

Authors - Peng Yang
View Article
humanWB

J Agric Food Chem

Cucurbitacin E Triggers Cellular Senescence in Colon Cancer Cells via Regulating the miR-371b-5p/TFAP4 Signaling Pathway.

Authors - Peng Yang
View Article
humanWB

Invest Ophthalmol Vis Sci

A Novel Small Peptide H-KI20 Inhibits Retinal Neovascularization Through the JNK/ATF2 Signaling Pathway.

Authors - Ruonan Wang
View Article