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验证数据展示

  • WB analysis of MCF-7 using 13183-1-AP

    WB analysis of MCF-7 using 13183-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13183-1-AP (TFAP2B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 13183-1-AP

    IP experiment of MCF-7 using 13183-1-AP

    IP result of anti-TFAP2B (IP:13183-1-AP, 4ug; Detection:13183-1-AP 1:500) with MCF-7 cells lysate 800ug.

TFAP2B Polyclonal antibody

TFAP2B Polyclonal Antibody for WB, IP, ELISA
Cat No. 13183-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

AP 2B, AP2 B, AP2 beta, TFAP2B, Transcription factor AP 2 beta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IP detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13183-1-AP targets TFAP2B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag3853

Product name: Recombinant human TFAP2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 111-460 aa of BC037225

Sequence: RQEVGSEAGSLLPQPRAALPQLSGLDPRRDYHSVRRPDVLLHSAHHGLDAGMGDSLSLHGLGHPGMEDVQSVEDANNSGMNLLDQSVIKKVPVPPKSVTSLMMNKDGFLGGMSVNTGEVFCSVPGRLSLLSSTSKYKVTVGEVQRRLSPPECLNASLLGGVLRRAKSKNGGRSLRERLEKIGLNLPAGRRKAANVTLLTSLVEGEAVHLARDFGYICETEFPAKAVSEYLNRQHTDPSDLHSRKNMLLATKQLCKEFTDLLAQDRTPIGNSRPSPILEPGIQSCLTHFSLITHGFGAPAICAALTALQNYLTEALKGMDKMFLNNTTTNRHTSGEGPGSKTGDKEEKHRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription factor AP-2 beta (activating enhancer binding protein 2 beta)
别名 AP 2B, AP2 B, AP2 beta, TFAP2B, Transcription factor AP 2 beta
计算分子量 449 aa, 49 kDa
观测分子量 50 kDa
GenBank蛋白编号BC037225
基因名称 TFAP2B
Gene ID (NCBI) 7021
RRIDAB_2286992
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92481
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TFAP2B antibody 13183-1-APDownload protocol
IP protocol for TFAP2B antibody 13183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncol Lett

miR-142-5p promotes renal cell tumorigenesis by targeting TFAP2B.

Authors - Maoshu Zhu
View Article