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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated TFAP2A,AP-2 Polyclonal antibody

TFAP2A,AP-2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-13019

Print datasheet

Synonyms

Activator protein 2, AP 2, AP 2 transcription factor, AP 2alpha, AP2, AP2 alpha, AP2TF, BOFS, FLJ51761, TFAP2, TFAP2A

验证数据展示

  • IF Staining of HepG2 using CL488-13019

    IF Staining of HepG2 using CL488-13019

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TFAP2A,AP-2 antibody (CL488-13019) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13019 targets TFAP2A,AP-2 in IF applications and shows reactivity with human samples.

Tested Applications IF/ICC
Tested Reactivity human
Immunogen TFAP2A,AP-2 fusion protein Ag4112 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
Synonyms Activator protein 2, AP 2, AP 2 transcription factor, AP 2alpha, AP2, AP2 alpha, AP2TF, BOFS, FLJ51761, TFAP2, TFAP2A
Calculated Molecular Weight 431 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC017754
Gene Symbol TFAP2A
Gene ID (NCBI) 7020
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05549
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The activator protein-2 (AP-2) family of transcription factors comprises five 52-kDa isoforms (AP-2α, AP-2β, AP-2γ, AP-2δ, and AP-2ε), which share a common structure: a proline/glutamine-rich transactivation domain in the N-terminal region and a helix-span-helix domain in the C-terminal region, which mediates dimerization and site-specific DNA binding. Depending on the cellular context, the AP-2 transcription factors are individually associated either with cell differentiation and development or with cancer progression/regression (PMID: 21966377). TFAP2A (AP-2-alpha) is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation (PMID: 11694877).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TFAP2A,AP-2 antibody CL488-13019Download protocol
Standard Protocols
Click here to view our Standard Protocols