验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, C6 cells, C2C12 cells, Caco-2 cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
82745-5-RR targets TFAM in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | transcription factor A, mitochondrial |
| 别名 | MtTF1, mtTFA, TCF6, TCF-6, Transcription factor 6-like 2 |
| 计算分子量 | 246 aa, 29 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | BC126366 |
| 基因名称 | TFAM |
| Gene ID (NCBI) | 7019 |
| RRID | AB_3743421 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q00059 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3, and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TFAM antibody 82745-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Appl Biochem Biotechnol Electrical Vagus Nerve Stimulation Ameliorates Cardiac Ischemia and Reperfusion Injury by Improving Mitochondrial Biogenesis Through the SIRT1/PGC-1α Pathway. | ||
J Ethnopharmacol Atractylodes macrocephala Koidz. water extract alleviates exercise-induced fatigue by activating mitochondrial biogenesis via the PGC-1α/NRF1/TFAM axis. | ||
Biomaterials A coenzyme-based "interface-welding" hydrogel coating for titanium implants promotes diabetic osseointegration by regulating mitochondrial dynamics. | ||
Foods Chemical Composition, Antioxidant Activity, Anti-Fatigue Function and Mechanism of Pomegranate Peel Polyphenols on Exercise-Induced Fatigue in Mice. | ||
Int J Pharm Isoquercitrin-loaded adipose-derived stem cell exosomes synchronize immunomodulation and neurovascular remodeling to accelerate spinal cord regeneration. | ||
Neuropeptides PACAP promotes mitochondrial biogenesis in rat primary chondrocytes via the SIRT1/PGC-1α pathway. |
