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TEX15 Polyclonal antibody

TEX15 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 24585-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 42, CT42, testis expressed 15, TEX15

验证数据展示

  • IHC staining of mouse testis using 24585-1-AP

    IHC staining of mouse testis using 24585-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24585-1-AP (TEX15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of rat testis using 24585-1-AP

    WB analysis of rat testis using 24585-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 24585-1-AP (TEX15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 24585-1-AP

    IP experiment of PC-3 using 24585-1-AP

    IP result of anti-TEX15 (IP:24585-1-AP, 4ug; Detection:24585-1-AP 1:300) with PC-3 cells lysate 3200ug.

经过测试的应用

Positive WB detected inrat testis tissue
Positive IP detected inPC-3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 24585-1-AP is TEX15 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen TEX15 fusion protein Ag20170 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name testis expressed 15
Synonyms Cancer/testis antigen 42, CT42, testis expressed 15, TEX15
Calculated Molecular Weight 2789 aa, 315 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC141841
Gene Symbol TEX15
Gene ID (NCBI) 56154
RRIDAB_2879623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXT5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TEX15 also named as Cancer/testis antigen 42 is a 2789 amino acid protein, which is expressed exclusively in testicular and cancerous tissues and may be involved in the development and metastasis of several different types of tumors. TEX15 exists as a 1279 amino acid protein in the mouse and rat testis. The molecular weight of mouse/rat TEX15 is 150 kDa (PMID: 26959739).

实验方案

Product Specific Protocols
WB protocol for TEX15 antibody 24585-1-APDownload protocol
IHC protocol for TEX15 antibody 24585-1-APDownload protocol
IP protocol for TEX15 antibody 24585-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Toxicol Lett

Downregulation of vdac2 inhibits spermatogenesis via JNK and P53 signalling in mice exposed to cadmium.

Authors - Yu Fang
View Article
humanWB

Fertil Steril

Differential expression of miR-23a/b-3p and its target genes in male patients with subfertility.

Authors - Masood Abu-Halima
View Article