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验证数据展示

  • IHC staining of mouse brain using 22612-1-AP (same clone as 22612-1-PBS)

    IHC staining of mouse brain using 22612-1-AP (same clone as 22612-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22612-1-AP (TET3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22612-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 22612-1-AP (same clone as 22612-1-PBS)

    IF Staining of U2OS using 22612-1-AP (same clone as 22612-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TET3 antibody (22612-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22612-1-PBS in a different storage buffer formulation.

TET3 Polyclonal antibody, PBS Only

TET3 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 22612-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

22612-1-PBS targets TET3 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18379

Product name: Recombinant human TET3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1184-1492 aa of BC022243

Sequence: KPDLHALHNSLSPAYGGAEFAELPSQAVPTDAHHPTPHHQQPAYPGPKEYLLPKAPLLHSVSRDPSPFAQSSNCYNRSIKQEPVDPLTQAEPVPRDAGKMGKTPLSEVSQNGGPSHLWGQYSGGPSMSPKRTNGVGGSWGVFSSGESPAIVPDKLSSFGASCLAPSHFTDGQWGLFPGEGQQAASHSGGRLRGKPWSPCKFGNSTSALAGPSLTEKPWALGAGDFNSALKGSPGFQDKLWNPMKGEEGRIPAAGASQLDRAWQSFGLPLGSSEKLFGALKSEEKLWDPFSLEEGPAEEPPSKGAVKEEK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tet oncogene family member 3
别名
计算分子量 1660 aa, 179 kDa
GenBank蛋白编号BC022243
基因名称 TET3
Gene ID (NCBI) 200424
RRIDAB_3085700
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO43151
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ten-eleven translocation protein 3 (TET3) belongs to the family of ten-eleven translocations (TETs) which induce DNA demethylation and gene regulation at the epigenetic level by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) (PMID: 31656201). In ovarian cancer, TET3 is reported as an oncogene or tumor suppressor during tumorigenesis and overexpressed in ovarian cancer tissues (PMID: 27141829).

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