验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse cerebellum tissue, HeLa cells, rat brain tissue |
| Positive IHC detected in | human colon cancer tissue, human pancreas cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy TESC Ready-To-Use IHC Kit. TESC primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 8 publications below |
产品信息
11125-1-AP targets TESC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TESC fusion protein Ag1596 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tescalcin |
| 别名 | TSC, Tescalcin, CHP3 |
| 计算分子量 | 25 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC015221 |
| 基因名称 | TESC |
| Gene ID (NCBI) | 54997 |
| RRID | AB_2201727 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96BS2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TESC antibody 11125-1-AP | Download protocol |
| IHC protocol for TESC antibody 11125-1-AP | Download protocol |
| IF protocol for TESC antibody 11125-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genome Biol Long non-coding RNA ROR decoys gene-specific histone methylation to promote tumorigenesis. | ||
J Neurosci CHP1-Mediated NHE1 Biosynthetic Maturation Is Required for Purkinje Cell Axon Homeostasis. | ||
Cancer Commun (Lond) Tescalcin is an unfavorable prognosis factor that regulats cell proliferation and survival in hepatocellular carcinoma patients.
| ||
Front Oncol The Expression Profile, Clinical Application and Potential Tumor Suppressing Mechanism of hsa_circ_0001675 in Head and Neck Carcinoma. | ||
Oncol Rep Differential expression of tescalcin by modification of promoter methylation controls cell survival in gastric cancer cells. | ||
BMC Cancer Tescalcin promotes highly invasive papillary thyroid microcarcinoma by regulating FOS/ERK signaling pathway. |
