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验证数据展示

  • WB analysis using 28153-1-AP

    WB analysis using 28153-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28153-1-AP (TENC1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 28153-1-AP

    IHC staining of human liver cancer using 28153-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28153-1-AP (TENC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 28153-1-AP

    IHC staining of human liver cancer using 28153-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28153-1-AP (TENC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TENC1 Polyclonal antibody

TENC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28153-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

C1 TEN, C1TEN, DKFZp686D13244, FLJ16320, KIAA1075, TENC1, Tensin 2, TNS2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28153-1-AP targets TENC1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 TENC1 fusion protein Ag27731 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tensin like C1 domain containing phosphatase (tensin 2)
别名 C1 TEN, C1TEN, DKFZp686D13244, FLJ16320, KIAA1075, TENC1, Tensin 2, TNS2
计算分子量 1419 aa, 154 kDa
观测分子量140-153 kDa
GenBank蛋白编号BC142668
基因名称 TENC1
Gene ID (NCBI) 23371
RRIDAB_3086032
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ63HR2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TENC1 antibody 28153-1-APDownload protocol
IHC protocol for TENC1 antibody 28153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols