• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TEKT3 Polyclonal antibody

TEKT3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12959-1-AP

Print datasheet

Synonyms

TEKT3, tektin 3

验证数据展示

  • IP experiment of mouse testis using 12959-1-AP

    IP experiment of mouse testis using 12959-1-AP

    IP result of anti-TEKT3 (IP:12959-1-AP, 4ug; Detection:12959-1-AP 1:1000) with mouse testis tissue lysate 3920 ug.

  • WB analysis of mouse testis using 12959-1-AP

    WB analysis of mouse testis using 12959-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12959-1-AP (TEKT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 12959-1-AP

    IHC staining of human testis using 12959-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12959-1-AP (TEKT3 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 12959-1-AP

    IHC staining of human testis using 12959-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12959-1-AP (TEKT3 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12959-1-AP targets TEKT3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen TEKT3 fusion protein Ag4080 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tektin 3
Synonyms TEKT3, tektin 3
Calculated Molecular Weight 490 aa, 57 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC031688
Gene Symbol TEKT3
Gene ID (NCBI) 64518
RRIDAB_2877898
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TEKT3 antibody 12959-1-APDownload protocol
IHC protocol for TEKT3 antibody 12959-1-APDownload protocol
IP protocol for TEKT3 antibody 12959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

Cell Mol Life Sci

Tektin bundle interacting protein, TEKTIP1, functions to stabilize the tektin bundle and axoneme in mouse sperm flagella

Authors - Xin-Yan Geng
View Article
mouseWB,IF

J Genet Genomics

Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice

Authors - Yaling Wang
View Article