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验证数据展示

  • WB analysis using 18085-1-AP

    WB analysis using 18085-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18085-1-AP (TEC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 18085-1-AP

    IHC staining of human lung cancer using 18085-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18085-1-AP (TEC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TEC Polyclonal antibody

TEC Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18085-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

PSCTK4, TEC, tec protein tyrosine kinase, Tyrosine protein kinase Tec

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, mouse kidney tissue, Jurkat cells, rat kidney tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18085-1-AP targets TEC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12804

Product name: Recombinant human TEC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 282-631 aa of BC101711

Sequence: TVSLYTKFGGEGSSGFRHYHIKETTTSPKKYYLAEKHAFGSIPEIIEYHKHNAAGLVTRLRYPVSVKGKNAPTTAGFSYEKWEINPSELTFMRELGSGLFGVVRLGKWRAQYKVAIKAIREGAMCEEDFIEEAKVMMKLTHPKLVQLYGVCTQQKPIYIVTEFMERGCLLNFLRQRQGHFSRDVLLSMCQDVCEGMEYLERNSFIHRDLAARNCLVSEAGVVKVSDFGMARYVLDDQYTSSSGAKFPVKWCPPEVFNYSRFSSKSDVWSFGVLMWEVFTEGRMPFEKYTNYEVVTMVTRGHRLYQPKLASNYVYEVMLRCWQEKPEGRPSFEDLLRTIDELVECEETFGR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tec protein tyrosine kinase
别名 PSCTK4, TEC, tec protein tyrosine kinase, Tyrosine protein kinase Tec
计算分子量 631 aa, 74 kDa
观测分子量 74 kDa
GenBank蛋白编号BC101711
基因名称 TEC
Gene ID (NCBI) 7006
RRIDAB_2878494
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42680
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TEC antibody 18085-1-APDownload protocol
WB protocol for TEC antibody 18085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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