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验证数据展示

  • WB analysis using 68464-1-Ig

    WB analysis using 68464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68464-1-Ig (TEAD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68464-1-Ig

    WB analysis using 68464-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68464-1-Ig (TEAD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of human placenta using 68464-1-Ig

    IF Staining of human placenta using 68464-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using TEAD3 antibody (68464-1-Ig, Clone: 2E6A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TEAD3 Monoclonal antibody

TEAD3 Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 68464-1-Ig

产品说明书

CloneNo. 2E6A12

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IF-P, ELISA

别名

DTEF 1, DTEF-1, ETFR 1, TEA domain family member 3, TEAD 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHCT 116 cells, JAR cells, HepG2 cells, U2OS cells, pig heart tissue
Positive IF-P detected inhuman placenta tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68464-1-Ig targets TEAD3 in WB, IF-P, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag4407

Product name: Recombinant human TEAD3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-324 aa of BC027877

Sequence: MNLDQVSKDKALQSMASMSSAQIVSASVLQNKFSPPSPLPQAVFSTSSRFWSSPPLLGQQPGPSQDIKPFAQPAYPIQPPLPPTLSSYEPLAPLPSAAASVPVWQDRTIASSRLRLLEYSAFMEVQRDPDTYSKHLFVHIGQTNPAFSDPPLEAVDVRQIYDKFPEKKGGLKELYEKGPPNAFFLVKFWADLNSTIQEGPGAFYGVSSQYSSADSMTISVSTKVCSFGKQVVEKVETEYARLENGRFVYRIHRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTSRDSQETLLVIAFVFEVSTSEHGAQHHVYKLVKD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 TEA domain family member 3
别名 DTEF 1, DTEF-1, ETFR 1, TEA domain family member 3, TEAD 3
计算分子量 435 aa, 49 kDa
观测分子量50 kDa
GenBank蛋白编号BC027877
基因名称 TEAD3
Gene ID (NCBI) 7005
RRIDAB_3085175
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ99594
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TEAD3, others named transcription enhancer factor 5(TEF5), is a member of the family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involvement in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in the placenta and choriocarcinoma cells(JEG-3) but is low in Hela, HepG2, and MCF-7.

实验方案

Product Specific Protocols
IF protocol for TEAD3 antibody 68464-1-IgDownload protocol
WB protocol for TEAD3 antibody 68464-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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