验证数据展示
经过测试的应用
| Positive WB detected in | BGC-823 cells, A375 cells, C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13283-1-AP targets TEAD1 in WB, IF, ELISA applications and shows reactivity with mouse, human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | mouse, human |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag4082 Product name: Recombinant human TEAD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-60 aa of BC026959 Sequence: MLTPSIVYECATRNFQRAPFTIWHFQRMFQPSKGQLFKVLVGFYPNLVEIGKADRGKEDE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TEA domain family member 1 (SV40 transcriptional enhancer factor) |
| 别名 | AA, NTEF 1, Protein GT IIC, REF1, TCF 13, TCF13, TEA domain family member 1, TEAD 1, TEAD1, TEF 1, TEF1, Transcription factor 13 |
| 计算分子量 | 426 aa, 47, 9 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC026959 |
| 基因名称 | TEAD1 |
| Gene ID (NCBI) | 7003 |
| RRID | AB_2287290 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P28347 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TEAD1 belongs to a protein family that share the TEA/ATTS domain. It acts as a transcriptional factor by binding to the GT-IIC and Sph(I 1 II) enhansons in the simian virus 40 (SV40) enhancer. Also it can modulate SV40 late transcription together with large T antigen. The transcriptional activity of TEAD1 required several regions of the proteins , a TBP-associated factors and a limiting transcriptional intermediary factors. TEAD1 also preforms an essential role in the Hippo signaling pathway. TEAD1 regulates gene expression of YAP1 and WWTR1/TAZ, thus mediating cell proliferation, migration and epithelial mesenchymal transition induction. TEAD is preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
发表文章
| Species | Application | Title |
|---|---|---|
Cell QKI shuttles internal m7G-modified transcripts into stress granules and modulates mRNA metabolism | ||
Adv Sci (Weinh) CRISPR/Cas13 sgRNA-Mediated RNA-RNA Interaction Mapping in Live Cells with APOBEC RNA Editing | ||
MedComm (2020) YAP/TEAD1 and β-catenin/LEF1 synergistically induce estrogen receptor α to promote osteogenic differentiation of bone marrow stromal cells | ||
Cancer Lett EGFR/c-myc axis regulates TGFβ/Hippo/Notch pathway via epigenetic silencing miR-524 in gliomas. | ||
Prog Orthod Mechanosensor YAP mediates bone remodeling via NF-κB p65 induced osteoclastogenesis during orthodontic tooth movement | ||
Int J Mol Sci Role of Clostridium perfringens Enterotoxin on YAP Activation in Colonic Sessile Serrated Adenoma/ Polyps with Dysplasia. |
