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验证数据展示

  • WB analysis of mouse testis using 25239-1-AP

    WB analysis of mouse testis using 25239-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25239-1-AP (TDRD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 25239-1-AP

    IP experiment of mouse testis using 25239-1-AP

    IP result of anti-TDRD5 (IP:25239-1-AP, 5ug; Detection:25239-1-AP 1:300) with mouse testis tissue lysate 2800ug.

  • IHC staining of mouse testis using 25239-1-AP

    IHC staining of mouse testis using 25239-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25239-1-AP (TDRD5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 25239-1-AP

    IHC staining of mouse testis using 25239-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25239-1-AP (TDRD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TDRD5 Polyclonal antibody

TDRD5 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 25239-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

RP11 427G13.1, TDRD5, tudor domain containing 5, TUDOR3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25239-1-AP targets TDRD5 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 TDRD5 fusion protein Ag18896 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tudor domain containing 5
别名 RP11 427G13.1, TDRD5, tudor domain containing 5, TUDOR3
计算分子量 981 aa, 110 kDa
观测分子量 ~116 kDa
GenBank蛋白编号BC130532
基因名称 TDRD5
Gene ID (NCBI) 163589
RRIDAB_2879981
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NAT2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TDRD5, also named as Tudor domain-containing protein 5, is a 981 amino acid protein, which contains one Tudor domain and three HTH OST-type domains. TDRD5 localizes in the cytiplasm and belongs to the TDRD5 family. TDRD5 is required during spermiogenesis to participate in the repression transposable elements and prevent their mobilization, which is essential for the germline integrity.

实验方案

Product Specific Protocols
WB protocol for TDRD5 antibody 25239-1-APDownload protocol
IHC protocol for TDRD5 antibody 25239-1-APDownload protocol
IP protocol for TDRD5 antibody 25239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols