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验证数据展示

  • WB analysis using 13370-1-AP (same clone as 13370-1-PBS)

    WB analysis using 13370-1-AP (same clone as 13370-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13370-1-PBS in a different storage buffer formulation.

  • IP experiment of U-937 using 13370-1-AP (same clone as 13370-1-PBS)

    IP experiment of U-937 using 13370-1-AP (same clone as 13370-1-PBS)

    IP result of anti-TDG (IP:13370-1-AP, 3ug; Detection:13370-1-AP 1:400) with U-937 cells lysate 3000ug. This data was developed using the same antibody clone with 13370-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13370-1-AP (same clone as 13370-1-PBS)

    IF Staining of HeLa using 13370-1-AP (same clone as 13370-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13370-1-AP (TDG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13370-1-PBS in a different storage buffer formulation.

  • WB analysis of human brain using 13370-1-AP (same clone as 13370-1-PBS)

    WB analysis of human brain using 13370-1-AP (same clone as 13370-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 13370-1-AP (TDG antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13370-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 13370-1-AP (same clone as 13370-1-PBS)

    WB analysis of HEK-293 using 13370-1-AP (same clone as 13370-1-PBS)

    WB result of TDG antibody (13370-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDG transfected HEK-293 cells. This data was developed using the same antibody clone with 13370-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TDG Polyclonal antibody, PBS Only

TDG Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 13370-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

EC:3.2.2.29, G/T mismatch-specific thymine DNA glycosylase, hTDG, thymine DNA glycosylase, Thymine-DNA glycosylase

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TDG Ready-To-Use IHC Kit. TDG primary antibody included.

产品信息

13370-1-PBS targets TDG in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4190

Product name: Recombinant human TDG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-410 aa of BC037557

Sequence: MEAENAGSYSLQQAQAFYTFPFQQLMAEAPNMAVVNEQQMPEEVPAPAPAQEPVQEAPKGRKRKPRTTEPKQPVEPKKPVESKKSGKSAKSKEKQEKITDTFKVKRKVDRFNGVSEAELLTKTLPDILTFNLDIVIIGINPGLMAAYKGHHYPGPGNHFWKCLFMSGLSEVQLNHMDDHTLPGKYGIGFTNMVERTTPGSKDLSSKEFREGGRILVQKLQKYQPRIAVFNGKCIYEIFSKEVFGVKVKNLEFGLQPHKIPDTETLCYGMPSSSARCAQFPRAQDKVHYYIKLKDLRDQLKGIERNMDVQEVQYTFDLQLAQEDAKKMAVKEEKYDPGYEAAYGGAYGENPCSSEPCGFSSNGLIESVELRGESAFSGIPNGQWMTQSFTDQIPSFSNHCGTQEQEEESHA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 thymine-DNA glycosylase
别名 EC:3.2.2.29, G/T mismatch-specific thymine DNA glycosylase, hTDG, thymine DNA glycosylase, Thymine-DNA glycosylase
计算分子量 410 aa, 46 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC037557
基因名称 TDG
Gene ID (NCBI) 6996
RRIDAB_2271704
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13569
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TDG belongs to the TDG/mug DNA glycosylase family. TDG corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. RNF4 interacts with and requires the base excision repair enzymes TDG and APE1 for active demethylation (PMID:20696907). TDG is modified by SUMO-1 and SUMO-2/3.The molecular weight of non-modified TDG is 46 kDa and modified TDG is 55-60 kDa.

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