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CoraLite® Plus 488 Anti-Mouse TCR Beta (H57-597)

TCR Beta Monoclonal Antibody for FC, IF/ICC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC, IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

H57-597

Cat No : CL488-65106

Print datasheet

Synonyms

T cell receptor beta chain, TCR Beta, Tcrb, TCRβ, Tib

验证数据展示

  • FC experiment of C57BL/6 mouse splenocytes using CL488-65106

    FC experiment of C57BL/6 mouse splenocytes using CL488-65106

    1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse TCR Beta (CL488-65106, Clone: H57-597) or 0.25 ug CoraLite® Plus 488 Armenian Hamster IgG Isotype Control (PIP) (CL488-65210, Clone: PIP). Cells were not fixed.

  • IF Staining of mouse splenocytes using CL488-65106

    IF Staining of mouse splenocytes using CL488-65106

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse TCR beta (CL488-65106, Clone: H57-597) at dilution of 1:300.

经过测试的应用

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65106-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:150-1:600
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65106 targets TCR Beta in IF, FC applications and shows reactivity with Mouse samples.

Tested Applications FC, IF/ICC
Tested Reactivity Mouse
Immunogen Affinity purified TCR from mouse DO-11.10 cells 种属同源性预测
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Full Name T-cell receptor beta chain
Synonyms T cell receptor beta chain, TCR Beta, Tcrb, TCRβ, Tib
Gene Symbol Tcrb
Gene ID (NCBI) 21577
RRIDAB_2883180
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

T-cell receptor (TCR) consists of heterodimeric glycoproteins (TCR alpha/beta or gamma/delta) that demonstrate homology with immunoglobulins (PMID: 2144901). TCR beta is a member of the immunoglobulin superfamily and is expressed on alpha/beta TCR-bearing T cells and thymocytes. The TCR heterodimers are noncovalently associated with CD3. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta (PMID: 1826255). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TCR Beta antibody CL488-65106Download protocol
Standard Protocols
Click here to view our Standard Protocols