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验证数据展示

  • WB analysis of HUVEC using 66377-1-Ig

    WB analysis of HUVEC using 66377-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66377-1-Ig (TCN2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

TCN2 Monoclonal antibody

TCN2 Monoclonal Antibody for WB, ELISA
Cat No. 66377-1-Ig

产品说明书

CloneNo. 2G6D2

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, ELISA

别名

TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHUVEC cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy TCN2 Ready-To-Use IHC Kit. TCN2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66377-1-Ig targets TCN2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2825

Product name: Recombinant human TCN2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-400 aa of BC011239

Sequence: MRHLGAFLFLLGVLGALTEMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANWHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVREEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 transcobalamin II; macrocytic anemia
别名 TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II
计算分子量 400 aa, 44 kDa
观测分子量 43 kDa
GenBank蛋白编号BC011239
基因名称 TCN2
Gene ID (NCBI) 6948
RRIDAB_2881754
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP20062
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)

实验方案

Product Specific Protocols
WB protocol for TCN2 antibody 66377-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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