验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, mouse small intestine tissue, rat liver tissue, MCF-7 cells, U-87 MG cells |
| Positive IHC detected in | mouse small intestine tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:300-1:600 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12157-1-AP targets TCN2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, canine, cat |
| 免疫原 |
CatNo: Ag2825 Product name: Recombinant human TCN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC011239 Sequence: MRHLGAFLFLLGVLGALTEMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANWHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVREEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcobalamin II; macrocytic anemia |
| 别名 | D22S676, D22S750, TC 2, TC II, TC2, TCII, TCN2, Transcobalamin 2, Transcobalamin II |
| 计算分子量 | 400 aa, 44 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC011239 |
| 基因名称 | TCN2 |
| Gene ID (NCBI) | 6948 |
| RRID | AB_2303252 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P20062 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TCN2 (Transcobalamin-2) is a vitamin B12 (cobalamin; Cbl) binding and transport protein in plasma that facilitates the cellular uptake of the vitamin. TCN2 is secreted in a constitutive fashion in most cells. Plasma TCN2 levels have been reported to be elevated in a number of diverse human diseases. Defects in TCN2 are cause of transcobalamin-2 deficiency. (10714245)
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for TCN2 antibody 12157-1-AP | Download protocol |
| WB protocol for TCN2 antibody 12157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Immunohistochemical quantification of the cobalamin transport protein, cell surface receptor and Ki-67 in naturally occurring canine and feline malignant tumors and in adjacent normal tissues. | ||
Int J Biol Macromol CD320 expression and apical membrane targeting in renal and intestinal epithelial cells. | ||
Anticancer Res Immunohistochemical Quantification of the Vitamin B12 Transport Protein (TCII), Cell Surface Receptor (TCII-R) and Ki-67 in Human Tumor Xenografts. | ||
Mol Pharm Investigation of Receptor-Mediated Cyanocobalamin (Vitamin B(12)) Transport across the Inner Blood-Retinal Barrier Using Fluorescence-Labeled Cyanocobalamin |
