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TCFL5 Polyclonal antibody

TCFL5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 29404-1-AP

Print datasheet

Synonyms

CHA, Cha transcription factor, E2BP 1, E2BP1, Figlb, HPV 16 E2 binding protein 1, TCFL5

验证数据展示

  • WB analysis of mouse testis using 29404-1-AP

    WB analysis of mouse testis using 29404-1-AP

    Mouse testis tissue were subjected to SDS PAGE followed by western blot with 29404-1-AP (TCFL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 29404-1-AP

    IHC staining of mouse testis using 29404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29404-1-AP (TCFL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TCFL5 antibody included

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TCFL5 Ready-To-Use IHC Kit. TCFL5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29404-1-AP targets TCFL5 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Immunogen TCFL5 fusion protein Ag13479 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription factor-like 5 (basic helix-loop-helix)
Synonyms CHA, Cha transcription factor, E2BP 1, E2BP1, Figlb, HPV 16 E2 binding protein 1, TCFL5
Calculated Molecular Weight 483 aa, 52 kDa
Observed Molecular Weight 52-60 kDa
GenBank Accession NumberBC046933
Gene Symbol TCFL5
Gene ID (NCBI) 10732
RRIDAB_2918297
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UL49
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TCFL5 antibody 29404-1-APDownload protocol
IHC protocol for TCFL5 antibody 29404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell Signal

TCFL5 knockdown sensitizes DLBCL to doxorubicin treatment via regulation of GPX4

Authors - Xueying Lu
View Article