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验证数据展示

  • WB analysis using 89174-1-RR (same clone as 89174-1-PBS)

    WB analysis using 89174-1-RR (same clone as 89174-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 89174-1-RR (TCF7L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 89174-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 89174-1-RR (same clone as 89174-1-PBS)

    IP experiment of HEK-293 using 89174-1-RR (same clone as 89174-1-PBS)

    IP result of anti-TCF4/TCF7L2 (IP:89174-1-RR, 4ug; Detection:89174-1-RR 1:2000) with HEK-293 cells lysate 1800 ug. This data was developed using the same antibody clone with 89174-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 89174-1-RR (same clone as 89174-1-PBS)

    IHC staining of human colon using 89174-1-RR (same clone as 89174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 89174-1-RR (TCF4/TCF7L2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 89174-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TCF4/TCF7L2 antibody included
  • IHC staining of human colon using 89174-1-RR (same clone as 89174-1-PBS)

    IHC staining of human colon using 89174-1-RR (same clone as 89174-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 89174-1-RR (TCF4/TCF7L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 89174-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TCF4/TCF7L2 antibody included
  • Cytometric bead array sample test of MP03819-1

    Cytometric bead array sample test of MP03819-1

    The mean TCF4/TCF7L2 concentration was determined to be 162.3 ng/mL in Hela nuclear cell extract based on a 1.2 mg/mL extract load, 7.7 ng/mL in Hela cytoplasmic cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP03819-1

    Cytometric bead array standard curve of MP03819-1

    Cytometric bead array standard curve of MP03819-1, TCF4/TCF7L2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 89174-1-PBS. Detection antibody: 89174-2-PBS. Standard: Ag4729. Range: 1.25-80 ng/mL.

  • Affinity of 89174-1-RR

    Affinity of 89174-1-RR

    Biolayer interferometry (BLI) kinetic assays of 89174-1-RR against Human TCF4/TCF7L2 were performed. The affinity constant is 1.01 nM.

TCF4/TCF7L2 Recombinant monoclonal antibody, PBS Only (Capture)

TCF4/TCF7L2 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Cytometric bead array, Indirect ELISA
Cat No. 89174-1-PBS

产品说明书

CloneNo. 262241D1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Cytometric bead array, Indirect ELISA

别名

TCF7L2, HMG box transcription factor 4, hTCF 4, hTCF-4, T cell factor 4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TCF4/TCF7L2 Ready-To-Use IHC Kit. TCF4/TCF7L2 primary antibody included.

产品信息

89174-1-PBS targets TCF4/TCF7L2 as part of a matched antibody pair:

MP03819-1: 89174-1-PBS capture and 89174-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4729

Product name: Recombinant human TCF7L2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-355 aa of BC032656

Sequence: MPQLNGGGGDDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESETNQNSSSDSEAERRPPPRSESFRDKSRESLEEAAKRQDGGLFKGPPYPGYPFIMIPDLTSPYLPNGSLSPTARTYLQMKWPLLDVQAGSLQSRQALKDARSPSPAHIVSNKVPVVQHPHHVHPLTPLITYSNEHFTPGNPPPHLPADVDPKTGIPRPPHPPDISPYYPLSPGTVGQIPHPLGWQGQPVYPITTGGFRHPYPTALTVNASMSRFPPHMVPPHHTLHTTGIPHPAIVTPTVKQESSQSDVGSLHSSKHQDSKKEEEKKKPHIKKPLNAFMLYMKEMRAKVVAECTLKESAAINQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transcription factor 7-like 2 (T-cell specific, HMG-box)
别名 TCF7L2, HMG box transcription factor 4, hTCF 4, hTCF-4, T cell factor 4
计算分子量 619 aa, 68 kDa
观测分子量60, 75 kDa
GenBank蛋白编号BC032656
基因名称 TCF4
Gene ID (NCBI) 6934
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQB0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TCF7L2 encodes an HMG-box transcription factor and key effector of canonical Wnt/β-catenin signaling, regulating development and tissue homeostasis. It is the strongest genetic susceptibility locus for type 2 diabetes. Alternative splicing produces functionally distinct isoforms, and TCF7L2 variants have also been linked to Crohn's disease and cancer, indicating pleiotropic roles in disease.

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