验证数据展示
经过测试的应用
| Positive WB detected in | U2OS cells, HCT 116 cells, Caco-2 cells, A549 cells, PC-12 cells, SH-SY5Y cells, K-562 cells, Daudi cells |
Planning an IHC experiment? We recommend our IHCeasy TCF4 Ready-To-Use IHC Kit. TCF4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68607-1-Ig targets TCF4 in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | TCF4 fusion protein Ag17897 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | transcription factor 4 |
| 别名 | 1C9A1, bHLHb19, Class B basic helix-loop-helix protein 19, E2 2, ITF 2 |
| 计算分子量 | 671 aa, 72 kDa |
| 观测分子量 | 72 kDa; 68 kDa |
| GenBank蛋白编号 | BC125084 |
| 基因名称 | TCF4 |
| Gene ID (NCBI) | 6925 |
| RRID | AB_3085302 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P15884 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TCF4 antibody 68607-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |