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验证数据展示

  • IF Staining of mouse skeletal muscle using 22337-1-AP

    IF Staining of mouse skeletal muscle using 22337-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TCF4/ITF2 antibody (22337-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skeletal muscle using 22337-1-AP

    IF Staining of mouse skeletal muscle using 22337-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using TCF4/ITF2 antibody (22337-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 22337-1-AP

    WB analysis using 22337-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22337-1-AP (TCF4/ITF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis using 22337-1-AP

    WB analysis using 22337-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22337-1-AP (TCF4/ITF2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 22337-1-AP

    WB analysis of K-562 using 22337-1-AP

    WB result of TCF4/ITF2 antibody (22337-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCF4/ITF2 transfected K-562 cells.

  • IHC staining of human testis using 22337-1-AP

    IHC staining of human testis using 22337-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 22337-1-AP (TCF4/ITF2 Antibody) at dilution of 1:200.

    Ready-to-use IHC kits available with TCF4 antibody included
  • IP experiment of mouse lung using 22337-1-AP

    IP experiment of mouse lung using 22337-1-AP

    IP result of anti-TCF4/ITF2 (IP:22337-1-AP, 4ug; Detection:22337-1-AP 1:500) with mouse lung tissue lysate 4000ug.

  • Featured Product
  • KD/KO Validated

TCF4/ITF2 Polyclonal antibody

TCF4/ITF2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 22337-1-AP

产品说明书

引用文献(92)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (5)

应用

WB, IHC, IF-P, IP, ELISA and More (3)

别名

TCF4, bHLHb19, Class B basic helix-loop-helix protein 19, E2 2, ITF 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inCaco-2 cells, K-562 cells
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy TCF4 Ready-To-Use IHC Kit. TCF4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22337-1-AP targets TCF4/ITF2 in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, pig, chicken, bovine, drosophila
免疫原

CatNo: Ag17813

Product name: Recombinant human TCF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 407-560 aa of BC125084

Sequence: PSTAMPGGHGDMHGIIGPSHNGAMGGLGSGYGTGLLSANRHSLMVGTHREDGVALRGSHSLLPNQVPVPQLPVQSATSPDLNPPQDPYRGMPPGLQGQSVSSGSSEIKSDDEGDENLQDTKSSEDKKLDDDKKDIKSITRSRSSNNDDEDLTPE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transcription factor 4
别名 TCF4, bHLHb19, Class B basic helix-loop-helix protein 19, E2 2, ITF 2
计算分子量 671 aa, 72 kDa
观测分子量 72 kDa
GenBank蛋白编号BC125084
基因名称 TCF4
Gene ID (NCBI) 6925
RRIDAB_2879076
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDP15884
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription factor 4 (TCF4), also known as SEF2, ITF2, E2-2, and ME2, binds to the immunoglobulin enchancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription By similarity. Human TCF4 mRNA expression is particularly high in the brain. Usage of numerous 5' exons of the human TCF4 gene potentially yields in TCF4 protein isoforms with 18 different N-termini. In addition, the diversity of isoforms is increased by alternative splicing of several internal exons. Some isoforms contain a bipartite nuclear localization signal and are exclusively nuclear, whereas distribution of other isoforms relies on heterodimerization partners. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.TCF4 has several splicing isoforms that are expressed differentially in tissues and during cancer progression(15547706, 12796377). The scopel of molecular weight of several isoforms is about 60-90 kDa (21789225).

实验方案

Product Specific Protocols
IF protocol for TCF4/ITF2 antibody 22337-1-APDownload protocol
IHC protocol for TCF4/ITF2 antibody 22337-1-APDownload protocol
IP protocol for TCF4/ITF2 antibody 22337-1-APDownload protocol
WB protocol for TCF4/ITF2 antibody 22337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Signal Transduct Target Ther

B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin

Authors - Cefan Zhou
View Article
WB

Signal Transduct Target Ther

Serine/threonine/tyrosine kinase 1 drives pancreatic carcinogenesis via GSK3β sequestration-mediated Wnt/β-catenin pathway hyperactivation.

Authors - Cefan Zhou
View Article
mouseChIP

Nature

Thalamocortical transcriptional gates coordinate memory stabilization.

Authors - Andrea Terceros
  • KO Validated
View Article
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article

Science

Single-cell multi-cohort dissection of the schizophrenia transcriptome

Authors - W Brad Ruzicka
View Article
WB

Mol Cancer

The miR-23a/27a/24 - 2 cluster drives immune evasion and resistance to PD-1/PD-L1 blockade in non-small cell lung cancer

Authors - Hao Luo
View Article
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