验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, U-251 cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32030-1-AP targets TCF20 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36912 Product name: Recombinant human TCF20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 480-650 aa of NM_005650 Sequence: TPQKKTSKRPSSSKKADSCTNSEGSSQPEEQLKSPMAESLDGGCSSSSEDQGERVRQLSGQSTSSDTTYKGGASEKAGSSPAQGAQNEPPRLNASPAAREEATSPGAKDMPLSSDGNPKVNEKTVGVIVSREAMTGRVEKPGGQDKGSQEDDPAATQRPPSNGGAKETSHA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcription factor 20 (AR1) |
| 别名 | KIAA0292, Nuclear factor SPBP, Protein AR1, SPBP, SPRE-binding protein |
| 计算分子量 | 1960aa, 212 kDa |
| 观测分子量 | 250 kDa |
| GenBank蛋白编号 | NM_005650 |
| 基因名称 | TCF20 |
| Gene ID (NCBI) | 6942 |
| RRID | AB_3670174 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9UGU0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transcription Factor 20 (TCF20) is a transcriptional activator that binds to the regulatory region of MMP3 and thereby controls stromelysin expression. It stimulates the activity of various transcriptional activators such as JUN, SP1, PAX6 and ETS1, suggesting a function as a coactivator (PMID: 10995766). The calculated molecular weight of TCF20 is 211 kDa, but due to extensive modifications (Glycosylation, Sumoylation. Phosphorylation), the observed molecular weight is about 250 kda.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TCF20 antibody 32030-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
