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TCERG1 Polyclonal antibody

TCERG1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21858-1-AP

Print datasheet

Synonyms

CA150, TAF2S, TCERG1, Transcription factor CA150

验证数据展示

  • WB analysis using 21858-1-AP

    WB analysis using 21858-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21858-1-AP (TCERG1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 21858-1-AP

    IHC staining of human brain using 21858-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with TCERG1 antibody included
  • IHC staining of human brain using 21858-1-AP

    IHC staining of human brain using 21858-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with TCERG1 antibody included
  • IF Staining of HeLa using 21858-1-AP

    IF Staining of HeLa using 21858-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 21858-1-AP (TCERG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TCERG1 Ready-To-Use IHC Kit. TCERG1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21858-1-AP is TCERG1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen TCERG1 fusion protein Ag16482 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription elongation regulator 1
Synonyms CA150, TAF2S, TCERG1, Transcription factor CA150
Calculated Molecular Weight 1098 aa, 124 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC111727
Gene Symbol TCERG1
Gene ID (NCBI) 10915
RRIDAB_11183756
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14776
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TCERG1 antibody 21858-1-APDownload protocol
IHC protocol for TCERG1 antibody 21858-1-APDownload protocol
IF protocol for TCERG1 antibody 21858-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols