验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HeLa cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11993-1-AP targets TCEAL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2653 Product name: Recombinant human TCEAL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-159 aa of BC000809 Sequence: MDKPRKENEEEPQSAPKTDEERPPVEHSPEKQSPEEQSSEEQSSEEEFFPEELLPELLPEMLLSEERPPQEGLSRKDLFEGRPPMEQPPCGVGKHKLEEGSFKERLARSRPQFRGDIHGRNLSNEEMIQAADELEEMKRVRNKLMIMHWKAKRSRPYPI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcription elongation factor A (SII)-like 1 |
| 别名 | p21, pp21, SIIR, TCEA like protein 1, TCEAL1 |
| 计算分子量 | 159 aa, 21 kDa |
| 观测分子量 | 26-30 kDa |
| GenBank蛋白编号 | BC000809 |
| 基因名称 | TCEAL1 |
| Gene ID (NCBI) | 9338 |
| RRID | AB_2877814 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15170 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TCEAL1 is homologous to TFIIS, a conserved eukaryotic transcription elongation factor. The predicted 157-amino acid protein has a calculated molecular mass of 21 kD.On Western blots of mammalian cell extracts,it migrated as a 27-kD protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TCEAL1 antibody 11993-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
